Tube-Gel Digestion

Microplate digestion of minipreps from a single microcentrifuge tube.

Mass spectrometry compatible surfactant for optimized in-gel protein digestion.

Identification of proteins resolved by SDS-PAGE depends on robust in-gel protein digestion and efficient peptide extraction, requirements that are often difficult to achieve. A lengthy and laborious procedure is an additional challenge of protein identification in gel. We show here that with the use of the mass spectrometry compatible surfactant sodium 3-((1-(furan-2-yl)undecyloxy)carbonylamino...

An automated in-gel digestion/iTRAQ-labeling workflow for robust quantification of gel-separated proteins.

Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of larger 1D gel-derived gel bands with liquid handling work stations is less we...

Gel lubrication of the tracheal tube cuff reduces pulmonary aspiration.

BACKGROUND Leakage of fluid occurs along the longitudinal folds within the wall of an inflated high-volume, low-pressure cuff. Theoretically, lubrication of the cuff with a water-soluble gel might prevent aspiration by plugging the channels in the cuff wall. Pulmonary aspiration during anesthesia has been linked with postoperative pneumonia and during critical illness causes ventilator-associat...

Effect of Collagen Gel on Sciatic Nerve Regeneration in the Polyvinylidene Fluoride Tube

Background: The limited availability of donor sites for nerve grafting has continued to stimulate research toward finding suitable alternatives for autograft. The aim of this research was to study the effect of piezoelectric channel and collagen gel on nerve regeneration. Materials and methods: This research is an experimental study. Forty eight male rats (200-250 gr) were used. After axotomy...